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polyclonal goat nid1  (R&D Systems Hematology)
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Gene expression of Adamts1 and its substrates nidogens and versican in WT and Ats1-KO mice. (a) Graph representing mRNA fold change expression of Adamts1 in WT organs (n = 5), relative to aorta. (b) Graphs representing relative mRNA fold change expression of substrates <t>Nid1</t> and Nid2 in organs from WT (n = 5) and Ats1-KO (n = 5) mice. (c) Graphs representing relative mRNA fold change expression of substrate Vcan in organs from WT (n = 5) and Ats1-KO (n = 5) mice. All results in graphs are expressed as the median with s.e.m. and statistical significance ( * p < 0.05; **p < 0.01).
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goat anti nid1 polyclonal ab  (R&D Systems Hematology)
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Gene expression of Adamts1 and its substrates nidogens and versican in WT and Ats1-KO mice. (a) Graph representing mRNA fold change expression of Adamts1 in WT organs (n = 5), relative to aorta. (b) Graphs representing relative mRNA fold change expression of substrates <t>Nid1</t> and Nid2 in organs from WT (n = 5) and Ats1-KO (n = 5) mice. (c) Graphs representing relative mRNA fold change expression of substrate Vcan in organs from WT (n = 5) and Ats1-KO (n = 5) mice. All results in graphs are expressed as the median with s.e.m. and statistical significance ( * p < 0.05; **p < 0.01).
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Figure 2. Characterization of <t>Nid1</t> and Nid2 proteolysis and immunolocalization in the vasculature of tumor xenografts. (a) 100 lg of pro- tein extract from tumor xenografts were resolved and analyzed by WB for Nid1 (top panel), Nid2 (middle panel) and ADAMTS1 (bottom panel) using the relevant antibodies. (b) Confocal images (340 magnification) of control and ADAMTS1-overexpressing tumors were acquired from samples immunolabeled with CD31 (green) and Nid1 (red) antibodies. (c) Confocal images (340 magnification) were acquired from samples immunolabeled with CD31 (green) and Nid2 (red) antibodies. For this figure, control and ADAMTS1 correspond to groups “Control High” and “ADAMTS1 Low,” respectively. [Color figure can be viewed in the online issue, which is available at wileyonlinelibrary.com.]
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Gene expression of Adamts1 and its substrates nidogens and versican in WT and Ats1-KO mice. (a) Graph representing mRNA fold change expression of Adamts1 in WT organs (n = 5), relative to aorta. (b) Graphs representing relative mRNA fold change expression of substrates Nid1 and Nid2 in organs from WT (n = 5) and Ats1-KO (n = 5) mice. (c) Graphs representing relative mRNA fold change expression of substrate Vcan in organs from WT (n = 5) and Ats1-KO (n = 5) mice. All results in graphs are expressed as the median with s.e.m. and statistical significance ( * p < 0.05; **p < 0.01).

Journal: Scientific Reports

Article Title: ADAMTS1 protease is required for a balanced immune cell repertoire and tumour inflammatory response

doi: 10.1038/s41598-018-31288-7

Figure Lengend Snippet: Gene expression of Adamts1 and its substrates nidogens and versican in WT and Ats1-KO mice. (a) Graph representing mRNA fold change expression of Adamts1 in WT organs (n = 5), relative to aorta. (b) Graphs representing relative mRNA fold change expression of substrates Nid1 and Nid2 in organs from WT (n = 5) and Ats1-KO (n = 5) mice. (c) Graphs representing relative mRNA fold change expression of substrate Vcan in organs from WT (n = 5) and Ats1-KO (n = 5) mice. All results in graphs are expressed as the median with s.e.m. and statistical significance ( * p < 0.05; **p < 0.01).

Article Snippet: For additional immunofluorescence determinations, sections were incubated with the following antibodies: polyclonal rabbit CD31 (AB28364, Abcam), polyclonal goat Nid1 (AF2570, R&D), polyclonal rabbit anti-mouse Versican V1 (2701534, Millipore), polyclonal rabbit anti-mouse Versican DPE (Versikine) (Ab19345, Abcam).

Techniques: Gene Expression, Expressing

Figure 2. Characterization of Nid1 and Nid2 proteolysis and immunolocalization in the vasculature of tumor xenografts. (a) 100 lg of pro- tein extract from tumor xenografts were resolved and analyzed by WB for Nid1 (top panel), Nid2 (middle panel) and ADAMTS1 (bottom panel) using the relevant antibodies. (b) Confocal images (340 magnification) of control and ADAMTS1-overexpressing tumors were acquired from samples immunolabeled with CD31 (green) and Nid1 (red) antibodies. (c) Confocal images (340 magnification) were acquired from samples immunolabeled with CD31 (green) and Nid2 (red) antibodies. For this figure, control and ADAMTS1 correspond to groups “Control High” and “ADAMTS1 Low,” respectively. [Color figure can be viewed in the online issue, which is available at wileyonlinelibrary.com.]

Journal: International journal of cancer

Article Title: Contribution of ADAMTS1 as a tumor suppressor gene in human breast carcinoma. Linking its tumor inhibitory properties to its proteolytic activity on nidogen-1 and nidogen-2.

doi: 10.1002/ijc.28271

Figure Lengend Snippet: Figure 2. Characterization of Nid1 and Nid2 proteolysis and immunolocalization in the vasculature of tumor xenografts. (a) 100 lg of pro- tein extract from tumor xenografts were resolved and analyzed by WB for Nid1 (top panel), Nid2 (middle panel) and ADAMTS1 (bottom panel) using the relevant antibodies. (b) Confocal images (340 magnification) of control and ADAMTS1-overexpressing tumors were acquired from samples immunolabeled with CD31 (green) and Nid1 (red) antibodies. (c) Confocal images (340 magnification) were acquired from samples immunolabeled with CD31 (green) and Nid2 (red) antibodies. For this figure, control and ADAMTS1 correspond to groups “Control High” and “ADAMTS1 Low,” respectively. [Color figure can be viewed in the online issue, which is available at wileyonlinelibrary.com.]

Article Snippet: Immunofluorescence Immunofluorescence staining was performed with the following antibodies: polyclonal goat anti-human Nid1 (AF2570, R&D), polyclonal goat anti-human Nid2 (AF3385, R&D), polyclonal rabbit anti-human Collagen IV (AB6586, Abcam), polyclonal rabbit anti-human CD31 (AB28364, Abcam), polyclonal rabbit anti-human laminin (L9393, Sigma-Aldrich), monoclonal mouse anti-SMA (A2547, Sigma-Aldrich) and monoclonal rat anti-mouse Endomucin (SC65495, SCBT).

Techniques: Control, Immunolabeling

Figure 4. Differential processing of Nid1 in breast tumor and healthy samples. (a) 50 lg of protein extract from tumor and healthy breast samples were resolved and evaluated by WB with a Nid1 antibody. Black arrow points to full length (FL) protein and gray arrow to fragments of Nid1. (b) 50 lg of protein extract of paired healthy and tumor samples were resolved and analyzed by WB with Nid1 antibody as previously indicated. (c) Graph repre- sents the quantification of WB images. The mean of each group is calculated (tumors: n 5 7; healthy tissue: n 5 6) and represented as % of full length or fragments (considering %FL 1 %Frag 5 100).p-value was calculated using the Student’s t-test (**, p < 0.01)

Journal: International journal of cancer

Article Title: Contribution of ADAMTS1 as a tumor suppressor gene in human breast carcinoma. Linking its tumor inhibitory properties to its proteolytic activity on nidogen-1 and nidogen-2.

doi: 10.1002/ijc.28271

Figure Lengend Snippet: Figure 4. Differential processing of Nid1 in breast tumor and healthy samples. (a) 50 lg of protein extract from tumor and healthy breast samples were resolved and evaluated by WB with a Nid1 antibody. Black arrow points to full length (FL) protein and gray arrow to fragments of Nid1. (b) 50 lg of protein extract of paired healthy and tumor samples were resolved and analyzed by WB with Nid1 antibody as previously indicated. (c) Graph repre- sents the quantification of WB images. The mean of each group is calculated (tumors: n 5 7; healthy tissue: n 5 6) and represented as % of full length or fragments (considering %FL 1 %Frag 5 100).p-value was calculated using the Student’s t-test (**, p < 0.01)

Article Snippet: Immunofluorescence Immunofluorescence staining was performed with the following antibodies: polyclonal goat anti-human Nid1 (AF2570, R&D), polyclonal goat anti-human Nid2 (AF3385, R&D), polyclonal rabbit anti-human Collagen IV (AB6586, Abcam), polyclonal rabbit anti-human CD31 (AB28364, Abcam), polyclonal rabbit anti-human laminin (L9393, Sigma-Aldrich), monoclonal mouse anti-SMA (A2547, Sigma-Aldrich) and monoclonal rat anti-mouse Endomucin (SC65495, SCBT).

Techniques:

Figure 6. Immunolocalization of nidogens in the vascular BM of healthy and tumor counterparts. (a) Confocal images (340 magnification) were acquired from samples immunolabeled with CD31 (green) and Nid1 (red) antibodies. Colocalization analysis was obtained by ImageJ software processing. White dots represent the colocalization of both CD31 and Nid1 signals. (b) Confocal images (340 magnification) were acquired from samples immunolabeled with CD31 (green) and Nid2 (red) antibodies. Colocalization analysis was obtained by ImageJ soft- ware processing as in (a). [Color figure can be viewed in the online issue, which is available at wileyonlinelibrary.com.]

Journal: International journal of cancer

Article Title: Contribution of ADAMTS1 as a tumor suppressor gene in human breast carcinoma. Linking its tumor inhibitory properties to its proteolytic activity on nidogen-1 and nidogen-2.

doi: 10.1002/ijc.28271

Figure Lengend Snippet: Figure 6. Immunolocalization of nidogens in the vascular BM of healthy and tumor counterparts. (a) Confocal images (340 magnification) were acquired from samples immunolabeled with CD31 (green) and Nid1 (red) antibodies. Colocalization analysis was obtained by ImageJ software processing. White dots represent the colocalization of both CD31 and Nid1 signals. (b) Confocal images (340 magnification) were acquired from samples immunolabeled with CD31 (green) and Nid2 (red) antibodies. Colocalization analysis was obtained by ImageJ soft- ware processing as in (a). [Color figure can be viewed in the online issue, which is available at wileyonlinelibrary.com.]

Article Snippet: Immunofluorescence Immunofluorescence staining was performed with the following antibodies: polyclonal goat anti-human Nid1 (AF2570, R&D), polyclonal goat anti-human Nid2 (AF3385, R&D), polyclonal rabbit anti-human Collagen IV (AB6586, Abcam), polyclonal rabbit anti-human CD31 (AB28364, Abcam), polyclonal rabbit anti-human laminin (L9393, Sigma-Aldrich), monoclonal mouse anti-SMA (A2547, Sigma-Aldrich) and monoclonal rat anti-mouse Endomucin (SC65495, SCBT).

Techniques: Immunolabeling, Software